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Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for… Click to show full abstract
Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
Keywords:
brains zebrafish;
microscopy;
microscopy larval;
larval zebrafish;
expansion microscopy
Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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recommendations!