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Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells.

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Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize… Click to show full abstract

Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain their localization using fluorescence microscopy.

Keywords: protein interactions; protein protein; fluorescence complementation; protein; bimolecular fluorescence

Journal Title: Methods in molecular biology
Year Published: 2022

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