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Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly… Click to show full abstract
Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing.
Keywords:
dna;
profiling protein;
targeted damid;
cell type;
type specifically;
biology
Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!