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Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study… Click to show full abstract
Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and how to analyze the process using Western blot or immunofluorescence based assays. The function of proteins can be conveniently assayed by high-efficient antibody mediated depletion.
Keywords:
xenopus egg;
nuclear pore;
egg extract;
pore complex
Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!