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Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice.

Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through… Click to show full abstract

Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through the plasma compartment for delivery to the liver for excretion. Here, we describe an in vivo RCT method that incorporates these steps, measuring movement of cholesterol from macrophages to the plasma, the liver, and finally to the feces in mice.

Keywords: cholesterol transport; reverse cholesterol; cholesterol; vivo

Journal Title: Methods in molecular biology
Year Published: 2017

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