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Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using… Click to show full abstract
Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.
Keywords:
acid schiff;
diastase;
periodic acid;
pas
Journal Title: Methods in molecular biology
Year Published: 2017
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2017
Link to full text (if available)
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recommendations!