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Argonaute (AGO) proteins play a key role in RNA silencing mechanisms. RNA silencing affects both RNA degradation and translation. The characterization of translation-associated RNA silencing mechanisms and components often requires… Click to show full abstract
Argonaute (AGO) proteins play a key role in RNA silencing mechanisms. RNA silencing affects both RNA degradation and translation. The characterization of translation-associated RNA silencing mechanisms and components often requires polysome isolation and analysis. In this chapter, we describe the identification of AGO1 association with polysomes through polysome fractionation on sucrose gradient, preparation of proteins by filtration and concentration, and immunoblotting.
Keywords:
association polysomes;
detection argonaute;
argonaute;
rna silencing;
argonaute association
Journal Title: Methods in molecular biology
Year Published: 2017
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Journal Title: Methods in molecular biology
Year Published: 2017
Link to full text (if available)
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recommendations!