B-1a cells are a unique subset of B cells that are phenotypically and functionally distinguishable from conventional (B-2) cells. Here we detail methods to analyze signaling events in B-1a cells.… Click to show full abstract
B-1a cells are a unique subset of B cells that are phenotypically and functionally distinguishable from conventional (B-2) cells. Here we detail methods to analyze signaling events in B-1a cells. We demonstrate by flow cytometry and Western blot that mouse peritoneal B-1a but not splenic B-2 cells have constitutive activation of signal transducers and activators of transcription-3 (Stat3) and extracellular signal-regulated kinase (Erk).
               
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