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Paper-based devices chemically functionalized with capturing molecules enable the isolation and characterization of extracellular vesicles (EVs) from samples of limited amount. Here, we describe the isolation of EV subpopulations from… Click to show full abstract
Paper-based devices chemically functionalized with capturing molecules enable the isolation and characterization of extracellular vesicles (EVs) from samples of limited amount. Here, we describe the isolation of EV subpopulations from human serum samples. The morphology, content, and amount of captured EVs can be assessed using scanning electron microscopy (SEM ), transcriptome analysis, and paper-based enzyme-linked immunosorbent assays (pELISA), respectively. A colorimetric readout can be detected from 10 μL serum within 10 min.
Journal Title: Methods in molecular biology
Year Published: 2017
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