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In Vitro Translation Assays for Selenocysteine Insertion.

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The molecular characterization of the protein and RNA factors that are required for Sec incorporation in mammals has been largely carried out using in vitro translation systems specifically modified for… Click to show full abstract

The molecular characterization of the protein and RNA factors that are required for Sec incorporation in mammals has been largely carried out using in vitro translation systems specifically modified for this purpose. This chapter outlines the various systems and modifications that have been used to decipher the mechanism of Sec incorporation.

Keywords: assays selenocysteine; vitro translation; translation; biology; selenocysteine insertion; translation assays

Journal Title: Methods in molecular biology
Year Published: 2018

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