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Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers.

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ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation… Click to show full abstract

ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.

Keywords: chip seq; sequencing chip; chip; native chromatin; chromatin immunoprecipitation; immunoprecipitation sequencing

Journal Title: Methods in molecular biology
Year Published: 2018

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