Photo from archive.org
Lyme disease Borreliae produces outer surface adhesins to confer bacterial attachment to the extracellular matrix (ECM) components on the surface of mammalian cells. Here, we describe protocols to characterize the… Click to show full abstract
Lyme disease Borreliae produces outer surface adhesins to confer bacterial attachment to the extracellular matrix (ECM) components on the surface of mammalian cells. Here, we describe protocols to characterize the activity and specificity of these adhesins by flow cytometry or measurement of the binding of radiolabeled spirochetes to immobilized ECM or mammalian cells.
Keywords:
characterization borrelia;
binding mammalian;
extracellular matrix;
mammalian cells;
burgdorferi binding;
borrelia burgdorferi
Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!
Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!