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Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells.

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Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4… Click to show full abstract

Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.

Keywords: human naive; cd4 cells; cells investigate; investigate global; naive cd4

Journal Title: Methods in molecular biology
Year Published: 2018

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