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Preparation of Solutions and Reagents.

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The ability to prepare accurate solutions, reagents, and buffers will determine the outcome of a successful experiment. Much time and effort spent in a lab will consist of making solutions,… Click to show full abstract

The ability to prepare accurate solutions, reagents, and buffers will determine the outcome of a successful experiment. Much time and effort spent in a lab will consist of making solutions, reagents, and buffers. This chapter introduces and refreshes the skill of making solutions including: molarity (M) concentration, percentage (%) concentration, mass/volume concentrations, X-factor concentration, working concentrations from stock solutions, conversions from percentage (%) to molarity (M) concentration, and conversions from molarity (M) to percentage (%) concentration. Basic formulas and examples for how to make solution concentrations are covered.

Keywords: molarity; preparation solutions; biology; percentage; solutions reagents; concentration

Journal Title: Methods in molecular biology
Year Published: 2018

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