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LC-MS/MS Analysis of Bile Acids.

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Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism… Click to show full abstract

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

Keywords: analysis bile; bile acids; biology

Journal Title: Methods in molecular biology
Year Published: 2018

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