Diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in 3-dimensional Gelfoam® histoculture for long periods of time and still maintain many of their in… Click to show full abstract
Diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in 3-dimensional Gelfoam® histoculture for long periods of time and still maintain many of their in vivo properties. The in vivo properties maintained in vitro include 3-dimensional growth; maintenance of tissue organization and structure, such as changes associated with oncogenic transformation; retention of differentiated function; tumorigenicity; and growth of multiple types of cells from a single tumor.
               
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