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Programming Biomaterial Interactions Using Engineered Living Cells.

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We have developed a biomaterials interface that allows the properties of a functionalized surface to be controlled by a population of genetically engineered bacteria. This interface was engineered by linking… Click to show full abstract

We have developed a biomaterials interface that allows the properties of a functionalized surface to be controlled by a population of genetically engineered bacteria. This interface was engineered by linking a genetically modified E. coli strain with a chemically functionalized surface. Critically, the E. coli was engineered to upregulate the production of biotin when induced by a small signaling molecule. This biotin would then interact with the functionalized surface to modulate the surface's binding dynamics. In this chapter, we detail three protocols: one protocol for developing a population of biotin-producing genetically engineered cells, and two protocols for creating different types of functionalized surfaces. These methods will enable scientists to readily explore strategies for controlling surface-based material assembly and modification using a linked culture of engineered cells.

Keywords: biomaterial interactions; interactions using; surface; functionalized surface; using engineered; programming biomaterial

Journal Title: Methods in molecular biology
Year Published: 2018

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