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Mass Spectrometry-Based Proteomics.

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Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols… Click to show full abstract

Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification.

Keywords: spectrometry; mass spectrometry; analysis; based proteomics; biology; spectrometry based

Journal Title: Methods in molecular biology
Year Published: 2018

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