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Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening… Click to show full abstract
Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.
Keywords:
mediated transformation;
transformation efficiency;
agrobacterium mediated;
transformation
Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!