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Precise Quantification of Platelet Proteins and Their Phosphorylation States.

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Systems biology and modeling approaches require quantitative data-rich temporal experiments to better understand the dynamics and regulation of the components of the signaling pathways that governs cell biology and physiology.… Click to show full abstract

Systems biology and modeling approaches require quantitative data-rich temporal experiments to better understand the dynamics and regulation of the components of the signaling pathways that governs cell biology and physiology. Here we present a modified Western blotting method to rapidly analyze and accurately quantify protein copy number, and their respective phosphorylation states at specific sites over detailed time courses.

Keywords: precise quantification; platelet proteins; phosphorylation states; quantification platelet; biology

Journal Title: Methods in molecular biology
Year Published: 2018

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