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The culture of the olfactory epithelium has been a useful model for the study of neurogenesis since olfactory neurons regenerate continuously throughout the adult lifespan. Structurally and functionally mature olfactory… Click to show full abstract
The culture of the olfactory epithelium has been a useful model for the study of neurogenesis since olfactory neurons regenerate continuously throughout the adult lifespan. Structurally and functionally mature olfactory neurons are generated in vitro from non-neuronal precursors in a process that resembles the in vivo counterparts. This chapter describes a technique for culture of olfactory neurons from the avian olfactory epithelium of embryonic chickens; this enables the controlled laboratory study of a critical sensory system that is unstudied in this major vertebrate class. The techniques described here are broadly applicable to other endothermic vertebrate species.
Journal Title: Methods in molecular biology
Year Published: 2018
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