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A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium… Click to show full abstract
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
Keywords:
bioencapsulation oocytes;
granulosa cells;
oocytes granulosa;
biology;
culture
Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!