LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Polarizing Region Tissue Grafting in the Chick Embryo Limb Bud.

Photo from wikipedia

The polarizing region of the developing limb bud is an important organizing center that is involved in anteroposterior (thumb to little finger) patterning and has three main functions that are… Click to show full abstract

The polarizing region of the developing limb bud is an important organizing center that is involved in anteroposterior (thumb to little finger) patterning and has three main functions that are now considered to depend on the secreted protein Sonic hedgehog (Shh). These are (1) specifying anteroposterior positional values by autocrine and graded paracrine signaling; (2) promoting growth in adjacent mesenchyme; (3) maintaining the distal epithelium that is essential for limb outgrowth by induction of a factor in adjacent mesenchyme. The polarizing region was identified using classical tissue grafting techniques in chicken embryos. Here we describe this procedure using tissue from transgenic Green Fluorescent Protein-expressing chicken embryos that allows the long-term fate of the polarizing region to be determined. This technique provides a highly useful and effective method to understand how the polarizing region patterns the limb and has implications for other organizing centers.

Keywords: tissue grafting; polarizing region; limb bud; region

Journal Title: Methods in molecular biology
Year Published: 2018

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.