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Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene… Click to show full abstract
Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene fragments in PCR reactions from AY-group phytoplasmas. Strain-identification was possible after electrophoresis and direct sequencing was also possible after PCR. The combinations of primers can be easily modified, so this method could be applied to other phytoplasma strains.
Keywords:
method;
pcr;
group phytoplasmas;
multiplex pcr;
pcr method
Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!