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Basic Antibacterial Assay to Screen for Bacteriocinogenic Lactic Acid Bacteria and to Elementarily Characterize Their Bacteriocins.

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Bacteriocins ribosomally produced by lactic acid bacteria are antibacterial peptides expected to be used as a safe biopreservative and a fermentation controller in food industry. The modified agar-well diffusion method… Click to show full abstract

Bacteriocins ribosomally produced by lactic acid bacteria are antibacterial peptides expected to be used as a safe biopreservative and a fermentation controller in food industry. The modified agar-well diffusion method is most frequently used for antibacterial activity assay to screen for potentially bacteriocin-producing strains and to elementarily characterize their bacteriocins and the relatives. Here, I describe procedure of the modified agar-well diffusion assay in the details.

Keywords: characterize bacteriocins; elementarily characterize; assay screen; acid bacteria; lactic acid

Journal Title: Methods in molecular biology
Year Published: 2019

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