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Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy.

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Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide… Click to show full abstract

Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.

Keywords: hepatitis virus; super resolution; resolution microscopy; microscopy

Journal Title: Methods in molecular biology
Year Published: 2019

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