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Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for… Click to show full abstract
Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for gene editing in mouse embryonic stem cells (mESCs) to generate knockout ESC chimeras for the fast-tracked analysis of gene function. Furthermore, we describe the application of gene editing directly to mouse epiblast stem cells (mEpiSCs) for modelling germ layer differentiation in vitro.
Keywords:
gene editing;
editing mouse;
mouse embryonic;
gene;
stem cells
Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!