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Phenotyping Biological Properties of CTV Isolates.

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The protocol described is intended to be used alongside molecular methods in order to reveal the relationship between the genome sequence and the biological properties of a single isolate of… Click to show full abstract

The protocol described is intended to be used alongside molecular methods in order to reveal the relationship between the genome sequence and the biological properties of a single isolate of Citrus tristeza virus complex (CTV). It enables the phenotypic profile of the isolates to be defined and to infer the associated tristeza diseases (decline, seedling yellows, or stem pitting), to assess their aggressiveness or potential cross protectiveness (if any), and to monitor their movement into the host plants and the transmissibility by aphids.

Keywords: biological properties; biology; phenotyping biological; properties ctv; ctv isolates

Journal Title: Methods in molecular biology
Year Published: 2019

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