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Using Aphids to Measure Turgor Pressure Inside Sieve Elements.

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The phloem is the long-distance transport system for photoassimilates within the plant. The vulnerability of the phloem tissue to blockage and damage makes it technically difficult to study, which has… Click to show full abstract

The phloem is the long-distance transport system for photoassimilates within the plant. The vulnerability of the phloem tissue to blockage and damage makes it technically difficult to study, which has made it one of the least understood tissues. Transport of solution through the phloem appears to be by osmotically driven bulk flow, making an understanding of phloem hydrostatic pressure important in our comprehension of phloem flow mechanics. Here we describe a method of making in vivo direct transient measurements of phloem hydrostatic pressure using excised aphid stylets to directly access the phloem.

Keywords: using aphids; phloem; turgor pressure; aphids measure; measure turgor; pressure

Journal Title: Methods in molecular biology
Year Published: 2019

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