Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using… Click to show full abstract
Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed in the vasculature using phloem specific promoters or constitutively expressed reference marker lines. Now, the majority of phloem cell types can be identified, allowing a precise cellular and subcellular localization of phloem proteins.
Keywords:
phloem;
live cell;
microscopy;
phloem proteins;
fluorescently tagged
Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!