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Common methods to quantify molds in the environment are based on the detection of viable and nonviable fungal components using cultivation technique or assessment by microscopy. These methods are time… Click to show full abstract
Common methods to quantify molds in the environment are based on the detection of viable and nonviable fungal components using cultivation technique or assessment by microscopy. These methods are time consuming and laborious and require a high expertise and especially in airborne exposure studies they showed poor reproducibility. Therefore alternative techniques based on molecular or immunological tools attract wide interest. The development of specific ELISAs based on polyclonal antibodies to detect mold antigens in airborne samples starting with the extraction of the antigen material up to evaluation of the sandwich ELISA is summarized in this chapter.
Journal Title: Methods in molecular biology
Year Published: 2019
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