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Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS.

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UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to… Click to show full abstract

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Keywords: stationary phase; using amide; amide stationary; amino acid

Journal Title: Methods in molecular biology
Year Published: 2019

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