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Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and… Click to show full abstract
Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.
Keywords:
p2x electrophysiology;
electrophysiology surface;
surface trafficking;
xenopus oocytes;
trafficking xenopus
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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recommendations!