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DNA stable isotope probing (DNA-SIP) allows for the identification of microbes that assimilate isotopically labeled substrates into DNA. Here we describe the analysis of sequencing data using the multiple window… Click to show full abstract
DNA stable isotope probing (DNA-SIP) allows for the identification of microbes that assimilate isotopically labeled substrates into DNA. Here we describe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). MW-HR-SIP has improved accuracy over other methods and is easily implemented on the statistical platform R. We also discuss key experimental parameters to consider when designing DNA-SIP experiments and how these parameters affect accuracy of analysis.
Keywords:
dna stable;
isotope probing;
dna;
analysis;
stable isotope;
sip
Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!