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Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.

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The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life… Click to show full abstract

The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows for monitoring virus replication in a spatial and temporal fashion. Here, we describe the multifluorescent live analysis of the multicompartment Herpes Simplex Virus Type-1 (HSV-1) by live cell confocal laser scanning microscopy.

Keywords: virus type; live analysis; herpes simplex; simplex virus; replication

Journal Title: Methods in molecular biology
Year Published: 2020

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