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This chapter reports the use of real-time quantitative PCR to detect Diplodia sapinea, a fungal plant pathogen that causes shoot tip dieback and tree mortality on pine trees. This molecular… Click to show full abstract
This chapter reports the use of real-time quantitative PCR to detect Diplodia sapinea, a fungal plant pathogen that causes shoot tip dieback and tree mortality on pine trees. This molecular approach represents a reliable and sensitive tool to detect fungal pathogens in DNA extracted from plant tissues and its use can be also recommended to study fungal behavior in host tissues by quantifying fungal growth in the latent phase, when symptoms in the host are not present yet.
Keywords:
quantitative pcr;
fungal plant;
time quantitative;
real time;
plant;
diplodia sapinea
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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recommendations!