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ATP Synthase: Expression, Purification, and Function.

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ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods… Click to show full abstract

ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purification, and functional assessment of E. coli ATP synthase.

Keywords: purification function; atp synthase; expression purification; synthase expression

Journal Title: Methods in molecular biology
Year Published: 2020

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