LAUSR

A Gram-negative, aerobic, and long rod-shaped bacterium, designated as H33E-04 T , was isolated from the soil of reclaimed land, Republic of Korea. The strain grew at a temperature range of 15–40 °C, pH 5.0–10.0, and 0–2% NaCl (w/v). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain H33E-04 T was in the same clade with Chitinophaga pinensis DSM 2588 T , Chitinophaga filiformis IFO 15056 T , and Chitinophaga ginsengisoli Gsoil 052 T with 98.4%, 97.9%, and 97.8% sequence similarities, respectively. The de novo genome assembly revealed that the DNA G + C content of the strain was 46.2 mol%. Comparative genome analysis between strain H33E-04 T and C. pinensis DSM 2588  T showed that the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 79.9% and 23.4%, respectively. The major respiratory quinone was menaquinone-7 (MK-7) and the predominant cellular fatty acids were iso-C 15:0 (31.7%), C 16:1 ω 5 c (31.2%), and iso-C 17:0 3-OH (11.8%), supporting the affiliation of strain H33E-04 T with the genus Chitinophaga . Based on phylogenetic, physiological, and chemotaxonomic characteristics, strain H33E-04 T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga agri sp. nov. is proposed. The type strain of Chitinophaga agri is H33E-04 T (= KACC 21303 T  = NBRC114512 T ).