LAUSR

A Gram-staining negative, motile, non-spore-forming, rod-shaped bacterium, designated NAJP-14T, was isolated from the alkali-saline soil in Heilongjiang, Northeast China. Phylogenetic analysis based on 16S rRNA gene sequencing illustrated that strain NAJP-14T was a member of the genus Pelagibacterium, and shared 94.6-96.6% sequence identities to species from the genus Pelagibacterium. Strain NAJP-14T grew at 20-45 °C (optimum, 30 °C), pH 7.0-10.0 (optimum, pH 8.0) and in the presence of up to 5% w/v NaCl. The menaquinone was determined to be Q (10). The major fatty acids were identified as C18:1w6c (38.7%), C16:0 (16.2%) and C19:0 cyclo w8c (13.9%). The G + C content of the genomic DNA was 61.2%. Out of the 3442 predicted genes, 3391 were protein-coding genes and 51 were ncRNA. Digital DNA-DNA hybridization (dDDH) estimation and average nucleotide identity (ANI) of the strain NAJP-14T and the type strains of related species in the same family ranged between 17.9 and 21.8% and between 61.4 and 78.7%, respectively. Based on these data, it is concluded that strain NAJP-14T possesses sufficient characteristics to differentiate it from all recognized Pelagibacterium species, and should be considered as a novel species for which the name Pelagibacterium limicola sp. nov. is proposed. The type strain is NAJP-14T (= CGMCC 1.16631T, = JCM 33746T).