LAUSR

Book’s topic High-performance liquid chromatography (HPLC) has been widely practiced for more than 50 years, and is now one of the most widespread analytical techniques. The practice of HPLC is no longer limited to chromatographers and specialists; on the contrary, it is quite ubiquitous, and is performed by thousands of practitioners worldwide, at many different levels: chemists, biologists, students, manufacturers, inspectors, etc. Progress in instrument performance and separation media has included the development of Bhyphenated^ techniques such as liquid chromatography– mass spectrometry, two-dimensional liquid chromatography, ultrahigh-pressure liquid chromatography, new stationary phases, and partially porous and sub-2-μm particles capable of delivering superior resolution, greater sensitivity, enhanced separation/identification power, and faster analysis. A number of dedicated conferences and exhibitions around the world have contributed to propel the global HPLC market, as driven by the rising awareness of consumers and potential practitioners. HPLC can now be regarded as a Bmature^ analytical technique that has contributed to analytical solutions in diverse fields, such as food science, pharmaceutical science, the life sciences, environmental science, and forensic science. However, without the proper choice of column sets and analytical conditions, as well as the availability of dedicated software, the development of any HPLC method can be an unrewarding and frustrating experience.