LAUSR

There is an increasing need for tools to monitor toxicity of contaminants of emerging concern (CECs) in wastewater. The purpose of this work was to assess interferences in the presence of total solids (TS) and total suspended solids (TSS) in the LuminoTox at concentrations typical of those found in municipal secondary effluent (SE) and to evaluate a simple sample enrichment method for increased CEC sensitivity. 4 or 10 µg/L atrazine in different TS concentrations and in corresponding filtrates (TSS removed) exhibited equivalent toxicities. Because the only difference between these two fractions is the TSS, this result demonstrates that, generally, this fraction does not induce toxicity nor interfere with the bioassay. At constant medium-low TS, the LuminoTox was able to detect the presence of 4 µg/L of atrazine but could not distinguish the change in atrazine concentration between 4 and 6 µg/L. No inhibition was observed in the presence of a mix of 14 CECs each at 0.23 µg/L. However, upon sample enrichment by lyophilization (50×), an inhibition of 81 ± 3% was observed. The enriched SE alone (not spiked with CECs) led to an inhibition of 49 ± 1%, indicating the detection of the CEC contribution to toxicity after sample preconcentration. The LuminoTox is a promising tool for monitoring SE; however, if the intent is to detect CECs, enrichment method optimization is required.