LAUSR

Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, China. Cryptosporidium spp. and Giardia duodenalis were detected by light and immunofluorescence microscopy and nested PCR (nPCR). Fifteen samples were positive (4.5%) by Kinyoun staining, 40 (11.6%) samples were positive by immunofluorescence test (IFT), and 39 (11.3%) samples were positive by nPCR for Cryptosporidium spp., Cryptosporidium bovis (11/39, 28.2%) was the most prevalent species, followed by C. ryanae (6/39, 15.4%), C. andersoni (5/39, 12.8%), C. struthionis (5/39, 12.8%), C. parvum (5/39, 12.8%), C. hominis (4/39, 10.3%) and C. canis (3/39, 7.7%). Thirteen out of 344 (3.8%) samples were positive for Giardia by simple microscopy, 20 (5.8%) by IFT and 18 samples (5.2%) yak faecal samples were Giardia positive by nPCR. Two G. duodenalis assemblages (B, E) were detected in this study. Nine positive samples for G. duodenalis assemblage E were from the towns of Xiewu (8/9, 4.9%) and Xiangda (1/9, 1.3%), and nine positive samples (9/9, 8.5%) for G. duodenalis assemblage B were from the town of Zhenqin. This report provides information about infection with Cryptosporidium species and G. duodenalis assemblages in domesticated 1–2-month-old highland yaks living in the Qinghai-Tibet Plateau region of China.