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EUROSPINE Meetings 2017: e-Poster Abstracts EUROSPINE 2017 Scientific Programme Poster presentations BASIC SCIENCE, BIOLOGY P1 BIOMINERAL CAP COATING REDUCES INFLAMMATORY BEHAVIOR OF PEEK IMPLANTS Leena Jongpaiboonkit, Nicholas Caras, William Murphy Tissue Regeneration Systems, Inc., Plymouth, USA Introduction: Prior research has suggested that the peri-inflammatory response around PEEK implants, also described as a ‘‘halo effect’’, may be due to the stimulation of specific inflammatory cytokines that enhance fibrous tissue formation [Olivares-Navarrete R, Spine, 2015 40(6):399–404]. The purpose of this study was to determine if a nanostructured calcium phosphate (CaP) coating on PEEK cylinders has the potential to reduce production of inflammatory mediators, and enhance production of anti-inflammatory mediators. This in vitro study examined hMSCs behaviors on PEEK and carbon fiber reinforced (CF) PEEK substrates with and without the nanostructured CaP coating. Specifically, we examined (1) osteoblastic differentiation and (2) secretion of inflammatory interleukins. Methods: A nanostructured CaP coating was applied to PEEK and CFPEEKcylinders through a proprietary biomimetic precipitation process. Materials were subjected to surface pre-treatments and subsequently incubated in a pH adjustedmineral solution. An incubation protocolwas followed for 4 days resulting in a continuous, plate-like nanostructure coating that provided high surface area favorable for cell attachment (image 1). Human mesenchymal stem cells were cultured on tissue culture polystyrene, PEEKandCF-PEEKwith andwithoutCaP coating. Osteoblastic differentiation and specific inflammatory cytokines that enhance fibrous tissue formation were assessed after 5 days. Results: The uncoated PEEK cylinders demonstrated relatively high levels of pro-inflammatory cytokines, specifically high levels of IL-1b (associated with fibrous tissue formation), and IL-1b, IL-6, and IL-8 (associated with chronic inflammation). These presence of the mineral coating eliminated the increases in pro-inflammatory cytokines. In addition, the coated PEEK cylinders promoted enhanced osteoblastic differentiation in comparison to uncoated PEEK cylinders. Conclusion: These results suggest that this CaP coating could reduce the production of specific inflammatory cytokines that enhance fibrous tissue formation, while enhancing osteoblastic differentiation on PEEK implants. This biologic response to coated PEEK and CF-PEEK potentially translates to a reduced potential for the ‘‘halo effect’’. Disclosures: Author 1: stock/shareholder: Tissue Regeneration Systems, Inc., employee: Tissue Regeneration Systems, Inc.. Author 3: stock/shareholder: Tissue Regeneration Systems. P2 DRUG X CAN INHIBIT ANTI-APOPTOTIC CELL DEATH INDUCED BY OXIDATIVE STRESS IN VITRO AND IMPROVE LOCOMOTOR FUNCTION OF LOWER LIMBS IN SPINAL CORD INJURY MODEL MICE IN VIVO Mikito Tsushima, Shiro Imagama, Kei Ando, Kazuyoshi Kobayashi Department of Orthopaedic Surgery, Nagoya University, Nagoya, Japan Introduction: Drug repositioning is a notable strategy that comprehensive screening of drugs enable to be clinically indicated for noncurable diseases. Thus, it is a way provided low cost and short term because drugs had been used generally and properly, and searched side effects in detail. The object of this study is to detect some drugs which we will be able to use clinically for spinal cord injury (SCI), by the drug repositioning strategy. Methods: In this study, we screened 1186 FDA-approved drugs to identify a clinically applicable small molecular medical compounds 123 Eur Spine J (2017) 26 (Suppl 2):S335–S405 DOI 10.1007/s00586-017-5225-1