LAUSR

Double-stranded RNAs from an isolate of Neofusicoccum luteum collected from grapevines were analyzed by high-throughput sequencing. Contig annotations revealed the presence of a potential novel virus belonging to the newly proposed family Fusariviridae. Completion of the viral genome sequence was performed. The genome is 6,244 nucleotide long, excluding the poly(A) tail and contains two putative open reading frames (ORFs). The first one encodes a large polypeptide of 1,552 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains typical of viral replicases. The second ORF encodes a putative 475-aa-long polypeptide showing weak homology to the corresponding ORF of Macrophomina phaseolina single-stranded RNA virus 1, for which no function is known so far. Phylogenetic analyses indicated that this virus should be considered a novel mycovirus belonging to the proposed family Fusariviridae, for which the name “Neofusicoccum luteum fusarivirus 1” (NlFV1) is proposed.