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Use of antibiotic disks to evolve drug-resistant bacteria

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The methods used to generate antibiotic-resistant bacterial strains can be labour-intensive, costly, lengthy and/or prone to plate-to-plate variation. We propose a simple, inexpensive and easily replicated method to expose bacteria… Click to show full abstract

The methods used to generate antibiotic-resistant bacterial strains can be labour-intensive, costly, lengthy and/or prone to plate-to-plate variation. We propose a simple, inexpensive and easily replicated method to expose bacteria to a continuous gradient of antibiotic concentration, providing an environment of positive selective pressure for evolution of antibiotic-resistant strains.

Keywords: disks evolve; drug resistant; evolve drug; resistant; antibiotic disks; use antibiotic

Journal Title: Antonie van Leeuwenhoek
Year Published: 2018

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