LAUSR

An 8-week feeding trial was conducted to evaluate the effects of three dietary methionine (Met) sources [dl-Met, coated-Met, and a methionine hydroxy analogue calcium salt (MHA-Ca)] for Chinese soft-shelled turtle (Pelodiscus sinensis). Triplicate groups of juvenile turtles (initial weight 3.48 ± 0.03 g) were fed twice per day at 3% of body weight with positive control diet (T1, 46% protein and 46% fish meal, FM), negative control diet (T2, 43% protein and 23% FM), or three other test diets supplemented with either 0.2% coated-Met (T3), 0.125% MHA-Ca (T4), or 0.1% dl-Met (T5) to the T2 basal formulation, respectively. The feeding trial was conducted in 15 350-L plastic containers with three replicates per dietary treatment. The results showed that the highest and lowest percentage weight gain (1023.5 ± 18.2 versus 882.1 ± 14.5%) and feed efficiency (87.2 ± 0.94 versus 81.4 ± 0.4%) were observed in turtles fed the T1 and T2 diet (P < 0.05), respectively. Significant improvement in weight gain was observed in turtles fed diets with Met supplementation, irrespective of source, compared with turtles fed the T2 diet. It was observed that dietary MHA-Ca had similar efficacy compared with dl-Met in terms of growth performance and feed utilization efficiency of the turtles (P > 0.05). Protein utilization efficiency was significantly higher in turtles fed T4 or T5 diet compared to the T2 diet. Whole body protein content (17.0 to 17.4% on a wet weight basis) in turtles fed with Met-added diets was comparable to those of turtles fed the T1 diet which were significantly higher compared to T2-fed turtles. Antioxidant defense system enzymes, superoxide dismutase, and glutathione peroxidase showed the highest activity, 658.8 ± 17.9 U/mL and 642.8 ± 17.5 μmol/L, respectively, in the serum of turtle fed the MHA-Ca supplemented diet and was significantly higher compared to turtles fed the T2 or T3 diet. The present results showed that P. sinensis are able to effectively use added MHA-Ca and dl-Met in low protein and low FM diets to enhance growth, feed utilization efficiency, nitrogen retention, and antioxidant defense system enzyme activities.