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Modification of the Francis Medium for the Conversion of Histoplasma capsulatum to the Yeast-Like Growth Phase

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We propose a modification of Francis agar used for identification of the causative agent of histoplasmosis by in vitro conversion of the mycelial culture to the yeast-like growth phase. For… Click to show full abstract

We propose a modification of Francis agar used for identification of the causative agent of histoplasmosis by in vitro conversion of the mycelial culture to the yeast-like growth phase. For improving of the growth characteristics of the medium, we used FT-agar with glucose-vitamin additives developed for culturing of the tularemia causative agent. The modified Francis medium is characterized by significantly higher growth properties and allowed 10-fold increasing the number of CFU of yeast-like cells of both American and African histoplasmosis causative agent.

Keywords: yeast like; medium; modification francis; like growth; growth

Journal Title: Bulletin of Experimental Biology and Medicine
Year Published: 2019

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