LAUSR

Peculiarities of biofilms formation by Campylobacter bacteria in mixed populations with other microbial contaminants was studied by real-time impedance spectroscopy on an automated xCelligence real time cell analyzer (RTCA). This method is based on measuring the medium resistance in special plates (E-plates) with interdigitated microelectrodes. Coculturing of campylobacter with coliform bacteria is accompanied by film formation; the intensity of this process varies depending on the type of the test cultures and the nature of their interaction in mixed populations. Film formation by C. jejuni during co-culturing with enterobacteria is maximum during the first hours and depends on the presence of stress factors in the environment. The biomatrix film was synthesized by 3 times more intensively in the presence of oxygen than in microaerobic conditions, and also by 1.7-4.3 times more active in the mixed culture with Enterobacter cloacae, E. coli, and K. pneumoniae. During co-culturing of campylobacter with salmonella, no enhanced film formation by the tested strains was observed. Unlike members of the genus Enterobacter intensively producing exopolysaccharides, pathogenic member of Enterobacteriaceae, salmonella, demonstrated weak capacity to form film matrix. The study of film formation by Campylobacter allows more accurate assessment of the effectiveness of sanitary bactericidal treatment of food industry facilities, predict the appearance of biofilms and the intensity of their formation depending son the nature of the antimicrobial effect and the used means.