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A Method of Direct Quantitation of Lactobacillus spp. in Intestinal Contents Based on Real-Time PCR.

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The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number… Click to show full abstract

The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.

Keywords: method direct; intestinal contents; quantitation lactobacillus; pcr; direct quantitation; lactobacillus spp

Journal Title: Bulletin of experimental biology and medicine
Year Published: 2021

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