ObjectivesTo improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase.ResultsPolyphosphate kinase was over-expressed in the bacterium. The… Click to show full abstract
ObjectivesTo improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase.ResultsPolyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained.ConclusionsThe recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.
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